In-silico Characterization of ICE1 Transcriptional Factor in Cold Signalling Network in Mungbean (Vigna radiata L.)

Aim: Mungbean (Vigna radiata L.) is a leguminous crop with high rich of protein and susceptible to cold stress. In silico characterization ICE1, tolerant transcriptional factor in Vigna regulatory network other species special refrence legume crops.
 Methodology: Genomic sequences radiata, V. angularis unguculata crops were retrieved from NCBI (https://pubmed.ncbi.nlm.nih.gov) used for multiple sequence alignment, Phylogenetic comparative analysis ICE1 motif analysis.
 Results: The present study showed that (VrICE1) gene was closely related VaICE1 angularis) then VuICE1 unguiculata). also revealed the bHLH domain region (247-298aa) low complexity co-localization signals V.radiataICE1. There similarities dissimilarities isoforms V.radiata. It noted VrICE1 had average hydropathicity (GRAVY) -0.593 one -0.6 other. An instability index 64.58 64.90. higher percentage non-polar amino acid content more number random coils followed by alpha helix secondary structure. there major involvement proteins biological molecular functions under stress.
 Conclusion: bioinformatics tools help researchers getting information regard functional aspect respect tolerance. A comprehensive different physical chemical properties could us identify their diversified usefulness.